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A non-chromatographic antibody purification method not relying on Protein A nor any ligand
About
A non-chromatographic antibody purification method not relying on Protein A nor any ligand, had been developed. Highly pure (>95%) human, mouse, rabbit or sheep IgG's are obtained in good yields within 20-30 minutes. The technology addresses a projected $440M antibody purification reagents market.
Key Benefits
The technology relies on unique detergent scaffolds onto which IgG's bind almost quantitatively, whereas the vast majority of non-IgG proteins are rejected. Bound IgG's are recovered from the detergent scaffolds in great purity (>95%) while leaving residual impurities behind. IgG purification was demonstrated in serum-free media containing BSA\HSA. The fact that, purified IgG's preserve their binding specificity, implies that, the presented method may provide a cost-effective viable alternative to: Protein A chromatography.
Applications
Antibody Purification