A real-time polymerase chain reaction (RT-PCR) assay that distinguishes between Burkholderia pseudomallei and Burkholderia mallei, based on single point mutation.

About

Description: The technology is a real-time polymerase chain reaction (RT-PCR) assay that distinguishes between Burkholderia pseudomallei and Burkholderia mallei, based on single point mutation. Using specific methods and kits, the assay involves the addition of oligonucleotides to mixtures containing nucleic acid isolated from samples and undergo nucleic acid amplification.

Potential applications: Clinical applications to differentiate Burkholderia species.

Benefits and advantages: Prior to this assay, an efficient and accurate test did not exist to distinguish between the two Burkholderia species, which are biothreat pathogens. Burkholderia pseudomallei and Burkholderia mallei are closely related pathogens that cause melioidosis and glanders, respectively. While melioidosis is a naturally occurring human disease, primarily in Asia and Australia, glanders is a naturally occurring infection in equines (horses) with incidental disease in humans.

Key Benefits

Benefits and advantages: Prior to this assay, an efficient and accurate test did not exist to distinguish between the two Burkholderia species, which are biothreat pathogens. Burkholderia pseudomallei and Burkholderia mallei are closely related pathogens that cause melioidosis and glanders, respectively. While melioidosis is a naturally occurring human disease, primarily in Asia and Australia, glanders is a naturally occurring infection in equines (horses) with incidental disease in humans.

Applications

Potential applications: Clinical applications to differentiate Burkholderia species.

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