Technology # 16-05 Technology related to Green Fluorescent Protein Manufacturing
When manufacturing proteins from bacteria that has DNA that has been engineered to produce a target protein, it is necessary to also engineer a tag onto the end of the target protein strand. The tag allows the target protein to be distinguished from the other non-target proteins when the bacteria are broken and the resulting proteins are separated. The tag that is used is Green Fluorescent Protein (GFP), and the target protein will always have the tag attached to it. To identify the target protein, a UV light is shown on the proteins and the one which glows green is the target. However, the cost to purify the target protein or peptide by removing the tag is a significant part of the cost of production. The method of separating the tag from the target utilizes protease, which is fairly expensive. Now there is a newly engineered form of GFP that is easy to separate from the target protein, allowing a large decrease in the cost to purify the target protein or peptide.
This Technology/Invention is available for licensing.
For interested parties seeking further information, feel free to contact: Mark Allen Lanoue Technology Manager / Tech Ventures University of Arkansas (479) 575-7243 [email protected]
Newly engineered form of GFP that is easy to separate from the target protein, allowing a large decrease in the cost to purify the target protein or peptide.
Application(s): Plasmid that harbors mutant gene Advantage(s): * Aids in purification * May be visually tracked during processing * Resists chemical cleavage and most endoproteases * Can be easily removed from end product consisting of a protein or peptide.