Register for free to
view the full innovation profile.
Several chimeric mice were obtained that transmitted the targeted allele through the germline. Offspring from the chimeric mice were then mated and the gene was deleted from them.
About
Abstract: JHU researcher generated mice carrying a targeted deletion of the Tll2 gene. The construct was generated which a LoxP site and a LoxP/neo cassette which were introduced into introns 6 and 7, respectively. Following homologous recombination in embryonic stem cells and transfer of the targeted cells into blastocysts, several chimeric mice were obtained that transmitted the targeted allele through the germline. Offspring from the chimeric mice were then mated with EIIa-cre transgenic mice to generate mice in which exon 7 had been completely deleted. Features: Mice homozygous for the deletion were viable and fertile. Mice homozygous for the Tll2 mutation exhibited slightly increased muscle weights compared to wild type mice. Stage of development: The animal was used to study TLL-2 role in regulating myostatin latency in vivo.