Non-invasive, pH insensitive glucose biosensor for diabetes patients.
The present invention provides a pH-insensitive glucose indicator protein/biosensor with intrinsic signal transduction mechanisms to provide non-invasive methods of monitoring a wide range of glucose concentrations in vivo. The glucose indicator protein (GIP) encompasses a glucose binding protein, a fluorescence donor, and a fluorescence acceptor. Conformational changes in the glucose binding protein caused by the glucose binding results in a change in fluorescent resonance energy between the fluorescent donor and acceptor, resulting in a detectable signal. Additionally, the GIP is insensitive to in vivo pH changes, but is sensitive to a wide range of glucose concentrations. Another aspect of the invention encompasses an in vivo method for the determining the concentration of glucose, by providing a biosensor comprising at least two pH insensitive GIPs.
pH insensitive. Sensitive to a wide range of glucose concentrations. Intrinsic transduction mechanisms. Provides for optical signal in the visible region upon glucose binding. Noninvasive for fast, painless, and convenient monitoring of glucose