Decellularized tissue-specific extracellular matrix and microenvironments for pancreatic islet organoid differentiation
This invention describes an innovative technology enabling the biofabrication of human islets from various cell sources including human pluripotent stem cells (HPSCs). A novel nondenaturing method of preparing decellularized tissue-specific extracellular matrix in combination with new growth factors induces self-assembly of pancreatic islet during differentiation of human pluripotent stem cells. The characterization of the organoids revealed that the organoids consisted of all major endocrine cell types, including α, β, δ, and pancreatic polypeptide cells that were assembled into a tissue architecture similar to that of human islets. These organoids are capable of secreting insulin and glucagon in response to glucose.
• Tissue matrix substrates prepared by a detergent-free method.
• Organoids contain all major endocrine cell types, including α, β, δ, and pancreatic polypeptide cells.
• These organoids are capable of secreting insulin and glucagon in response to glucose.