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Our method is fast, accurate, and objective and usually requires no extra hardware, being based on image acquisition which most laboratory microscopes are equipped with.
About
Summary The invention is an image processing method of light microscopy images for the purpose of quantifying density of cells in cultures . A grayscale image of a cell culture is digitally captured using a light microscope and a CCD camera. Our method evaluates the culture’s confluency using an image processing algorithm on the image which is able to differentiate between background medium and cultured cells. It provides a quantitative, objective, standard and reproducible confluency measure, it is non-invasive, and it employs the most basic equipment found in biology labs: an optical microscope.