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The microscope has ultrafast imaging speed – from 10-20 Hz to 1kHz full frame, a large FOV and 2D tilted foci array pattern and 1D scanning mechanism
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Summary Stanford researchers have developed an ultrafast multi-foci two-photon microscope system that aims at 1 kHz full frame rate with 500x500 μm2 field of view (FOV). It utilizes a 2D foci-array pattern and 1D scanning mechanism to achieve full FOV excitation coverage. A customized high-speed intensified CMOS camera is used for parallel detection of fluorescence signals from all laser foci. A custom image processing algorithm is then performed to reconstruct a complete image frame from its multiple sub-frames while correcting for optical cross talk between nearby foci. This invention advances state-of-the-art in vivo microscopy to match the recent progress of fluorescent voltage indicators with higher imaging speeds and large FOV.