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Dynamic biophysical methods are among the techniques that have been improved so that they can provide information about intracellular protein movements
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Summary The invention relates to applying dynamic biophysical methods that measure the motion of intracellular entities to identify the expression or activity of a protein in a cell. The method combines live cell imaging, labeling of specific sites in cells and analyzing results to identify the dynamics type. It involves single-particle tracking, a powerful technology that allows the characterization of dynamic processes on the single-molecule or single-particle level with nanometer spatial and sub-second temporal precision. The acquired dynamic data is thoroughly analyzed to provide information on cell vitality and other cellular characteristics. In particular, the approach enables measuring the dynamics of different entities in the nuclear compartment of living mammalian cells.