Purification Active Antibodies
Precipitation for Non-chromatographic Purification.
After purification, native binding activity is restored with the purified product containing only bivalently active antibodies.
An affinity-based precipitation method for the rapid and nonchromatographic purification of bivalently active monoclonal antibodies combines the selectivity of affinity chromatography with the simplicity of salt-induced precipitation. The process demonstrates the ability to purify antibodies from typical contaminants including CHO cell conditioned media, ascites fluid, DNA, and other antibodies with yields >85% and with >95% purity. Purified antibodies display native binding levels to cell lines expressing the target proteins. Selectivity of the affinity-based precipitation method for bivalent activity can be established by purifying solutions containing both active and chemically denatured antibodies. Prior to purification, solutions can displayed 20−76% reduction in binding activity, where after purification, native binding activity is restored with the purified product containing only bivalently active antibodies.
1.Reduced purification costs 2. Increased purified product quality 3. Affinity-based precipitation method does not adversely affect the antibodies 4. Rapid and straightforward process for purification of antibodies