Use of automated sampler allows multiple different combinations of glycolipids to be spotted in an efficient manner. Only small volumes of test solution are required.

About

Assessing binding of proteins and antibodies

Cell membranes are coated with a layer of sugar and fat molecules termed glycolipids. It is increasingly realised that glycolipids have important functions and many cell-cell interactions, adhesion, motility and signalling events are mediated by glycolipid interactions.

In Multiple Sclerosis and the inflammatory paralysis Guillain Barré syndrome, some patients have antibodies that react against their own glycolipids. Previously, such interactions and antibodies were studied by isolating single glycolipids and analysing binding by ELISA (Enzyme-linked immunosorbent assay).

The University of Glasgow has identified a novel way of looking at proteins that bind glycolipids in membranes in a new process called combinatorial glycorray. This glycoarray allows multiple complexes to be spotted onto an artificial membrane composed of polyvinyldifluoride (PVDF) which can then be used to assess binding of proteins and antibodies.

Key Benefits


Automations – use of automated sampler allows multiple different combinations of glycolipids to be spotted in an efficient manner
Small sample size – only small volumes of test solution are required
Hydrophobic attachment – PVDF membranes bind glycolipids via a hydrophobic interaction with their lipid tails. As such, on PVDF, these are displayed in keeping with their in vivo orientation


Applications


Screening for disease markers in autoimmune conditions such as Multiple Sclerosis and Guillain Barré syndrome
Identifying novel targets for bacterial cell and toxin binding
Screening antibodies for complex binding


IP Status

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