PVDF Glycoarray

About

Assessing binding of proteins and antibodies Cell membranes are coated with a layer of sugar and fat molecules termed glycolipids. It is increasingly realised that glycolipids have important functions and many cell-cell interactions, adhesion, motility and signalling events are mediated by glycolipid interactions. In Multiple Sclerosis and the inflammatory paralysis Guillain Barré syndrome, some patients have antibodies that react against their own glycolipids. Previously, such interactions and antibodies were studied by isolating single glycolipids and analysing binding by ELISA (Enzyme-linked immunosorbent assay). The University of Glasgow has identified a novel way of looking at proteins that bind glycolipids in membranes in a new process called combinatorial glycorray. This glycoarray allows multiple complexes to be spotted onto an artificial membrane composed of polyvinyldifluoride (PVDF) which can then be used to assess binding of proteins and antibodies. Key Benefits Automations – use of automated sampler allows multiple different combinations of glycolipids to be spotted in an efficient manner Small sample size – only small volumes of test solution are required Hydrophobic attachment – PVDF membranes bind glycolipids via a hydrophobic interaction with their lipid tails. As such, on PVDF, these are displayed in keeping with their in vivo orientation Applications Screening for disease markers in autoimmune conditions such as Multiple Sclerosis and Guillain Barré syndrome Identifying novel targets for bacterial cell and toxin binding Screening antibodies for complex binding IP Status This technology is available as an Easy Access licence deal to companies and individuals.