Single Cell Transfection
Single Cell Transfection and Secretome Detection.
This invention uses the same nanopore and applied nanopore voltage to transfect the cell making sing cell secretome detection possible.
Biomolecule detection is capable in a microfluidic environment through the use of a nanopore. Single Nanopores, the size of which can be accurately controlled, have the electrical current sensitivity required to characterize molecules passing through them. Charged molecules of similar size and weight do produce a measurable difference in the current signal as a cell translocates through the pore. This invention uses the same nanopore and applied nanopore voltage to transfect the cell making sing cell secretome detection possible. The secretome offers the opportunity to identify new biomarkers for disease diagnosis and a promising approach to drug discovery. However, these proteins are secreted in only minute quantities, making it very difficult to detect and analyze. Moreover, gene expression produces a heterogeneous population of cells in tissue requiring analysis of the secretome is carried out with single cell resolution. The use and control over nanopore geometries and their electrical potentials offers the ability to accomplish these tasks.
- Provides for the sensitive detection of small amount of charged molecules, i.e. proteins, secreted by a single cell. - Nanopore array configuration can be used for parallel processing - Secretome analysis and detection post transfection on a single cell basis can provide insight to disease progression and potential pharmaceutical efficacy.